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KMID : 1094720070120050497
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 5 p.497 ~ p.501
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Application of sodium propionate to the suspension culture of Chinese hamster ovary cells for enhanced production of follicle-stimulating hormone
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Yoon Sung-Kwan
Ahn Yong-Ho
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Abstract
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In this study, we found that adding sodium propionate to batch culture medium resulted in cell growth suppression in a dosedependent manner, while it enhanced follicle-stimulating hormone (FSH) production in Chinese hamster ovary (CHO) cells. In a pseudo-perfusion culture, with the exchange of fresh medium containing 2.5 mM sodium propionate, a final FSH of 532 μg was obtained, which was approximately 1.5-fold higher than the control. Overall, the results demonstrate that the application of sodium proplonate for the commercial production of recombinant proteins in rCHO cells is feasible.
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KEYWORD
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sodium propionate, CHO cells, follicle-stimulating hormone
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